Publications

Peer-Reviewed Publications

52. Glycosaminoglycans in tissue regeneration: insights into glycobiology and their biomedical application; A. Kasarinaite, J. Ou, K. Chen, D. Peng, W. Jia, C. Ding, W. Huang*, D. Hay*, Y. Chen*; Bioactive Materials, 2026/01/29, 60 320-337.

51. Genetically programmable protein-biomineral core-shell nanovectors for enhancing tumor microenvironment-activated chemotherapy; K Zhang, X Sun, T Ji, X Shen, Y Li, H Zhao, X Yang, H Li, W Huang*; Materials Today Bio, 2025/12/30, 36 102754.

50. Genetically Engineered Light-Responsive In Situ Hydrogels for Immunomodulation and Multimodal Therapy in Metastatic Triple-Negative Breast Cancer; X. Shen, J. Zhang, J. Ou, Z. Bai, T. Liu, K. Zhang, K. Zhu, J. Wang, C. Wang, Q. Zhang, L. Lu, W. Huang*; Advanced Science, 2025/10/29, 13(3) e12355

49. Ultrafast crosslinking, strongly adhesive de novo protein hydrogels promote cartilage regeneration; K. Nie, Z. Fan, W. Sun, B. Wu, S. Zhou, H. Zhao, J. Ou, T. Ji, J. Tian, W. Wei, H. Ouyang*, D. Hay*, W. Huang*; Bioactive Materials, 2025/10/27, 56 368-385. (Featured as Cover)

48. RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage; Z. Wang*, H.Yu*, Z. Gu, X. Shi, J. Ma, Q. Shao, Y. Yao, S. Yao, Y. Xu, Y. Gu, J. Dai, Q. Liu, J. Shi, R. Qi, Y. Jin, Y. Liu, X. Shen, W. Huang, H. Liu, M. Jin, W. Liu, M. Brook#, D. Chen#; Nature communications, 2025/05/23, 16 4792.

47. Electrochemical-Genetic Programming of Protein-Based Magnetic Soft Robots for Active Drug Delivery; H. Zhao, B. Yu, D. Yu, T. Ji, K. Nie, J. Tian, X. Shen, K. Zhang, J. Ou, X. Yang, D. Xiao, Q. Zhou, W. Huang*; Advanced Science, 2025/04/29, 12(27) 2503404. (Featured as Inside Front Cover)

46. Bioinspired genetic and chemical engineering of protein hydrogels for programable multi-responsive actuation; T. Ji, H. Shi, X. Yang, H. Li, D.L .Kaplan, J. Yeo, W. Huang*; Advanced Healthcare Materials, 2024/06/08, 13(27) 2401562. (Featured as Back Cover)

45. Compressive strength degradation of engineered bamboo subjected to fungal attack; Y. Xiao, C. Chen, S. Zhang, Y. Kong, T. Ji, W. Huang, Y. Hu, D. Zhang, Y. Xiao*; npj Materials Degradation, 2023/11/29, 7 92.

44. Triboelectric performances of biodegradable polymers; H. Meng, Q. Yu, Z. Liu, Y. Gai, J. Xue, Y. Bai, X. Qu, P. Tan, D. Luo, W. Huang, K. Nie, W. Bai, Z. Hou, R. Tang, H. Xu, Y. Zhang, Q. Cai, X. Yang, Z. Wang, Z. Li*; Matter, 2023/10/31, 6(12) 4274-4290.

43. Advanced silk materials for musculoskeletal tissue regeneration; K. Nie#, S. Zhou#, H. Li, J. Tian, W. Shen, W. Huang*; Frontiers in Bioengineering and Biotechnology, 2023/05/02, 11 1199507.

42. Dynamic photoelectrical regulation of ECM protein and cellular behaviors; X. Wang, C. Yao. X. Yao. J. Lin, R. Li, K. Huang, W. Lin, X. Long, C. Dai, J. Dong, X. Yu, W. Huang, W. Weng, Q. Wang, H. Ouyang*, K. Cheng*; Bioactive Materials, 2022/09/30, 22 168-179.

41. Thermo- and Ion-responsive Silk-Elastin-Like Proteins and Their Multiscale Mechanisms; H. Shi, T. Ji, C. Zhai, J. Lu, W. Huang*, J. Yeo*; Journal of Materials Chemistry B, 2022/07/25,10(32) 6133-6142.

40. Biomacromolecule-based agent for high-precision light-based 3D hydrogel bioprinting; Y. Ma, W. Wei, L. Gong, C. Li, Y. Hong, X. Wang, R. Liang, Q. Shao, Q. Liang, W. Huang, M. J. Shipston, H. Ouyang*; Cell Reports Physical Science, 2022/07/18, 3(8) 100985.

39. Engineering Natural and Recombinant Silks for Sustainable Biodevices; X. Shen, H. Shi, H. Wei, B. Wu, Q. Xia, J. Yeo, W. Huang*; Frontiers in Chemistry, 2022/05/05, 10 881028.

38. Biomimetic Joint Paint for Efficient Cartilage Repair by Simultaneously Regulating Cartilage Degeneration and Regeneration in Pigs; W. Wei, Y. Ma, X. Zhang, W. Zhou, H. Wu, J. Zhang, J. Lin, C. Tang, Y. Liao, C. Li, X. Wang, X. Yao, Y. W. Koh, W. Huang, H. Ouyang*; ACS Applied Materials & Interfaces, 2021/10/27, 13 (46) 54801-54816.

37. Rapid printing of bio-inspired 3D tissue constructs for skin regeneration; F. Zhou, Y. Hong, R. Liang, X. Zhang, Y. Liao, D. Jiang, J. Zhang, Z. Sheng, C. Xie, Z. Peng, X. Zhuang, V. Bunpetch, Y. Zou, W. Huang, Q. Zhang, E. Alakpa, S. Zhang*, H. Ouyang*; Biomaterials, 2020/08/14, 258 120287.

36. Stimuli-Responsive Composite Biopolymer Actuators with Selective Spatial Deformation Behavior; Y. Wang#, W. Huang#, Y. Wang, X. Mu, S. Ling, H. Yu, W. Chen, C. Guo, M.C. Watson, Y. Yu, L.D. Black III, M. Li, F.G. Omenetto, C. Li*, D. L. Kaplan*; Proceedings of the National Academy of Sciences of the United States of America, 2020/06/10, 117(25) 14602-14608.

35. Smart Material Hydrogel Transfer Devices Fabricated with Stimuli-Responsive Silk-Elastin-Like Proteins; R. Parker, D. Cairns, W. Wu, K. Jordan, C. Guo, W. Huang, Z. Martin‐Moldes, D. L. Kaplan*; Advanced Healthcare Materials, 2020/04/27, 9(11) 2000266.

34. 3D Printing of Silk Protein Structures by Aqueous Solvent-Directed Molecular Assembly; X. Mu, Y. Wang, C. Guo, Y. Li, S. Ling, W. Huang, P. Cebe, H. Hsu, F. Ferrari, X. Jiang, Q. Xu, A. Balduini, F.G. Omenetto, D. L. Kaplan*; Macromolecular Bioscience, 2019/08/21, 20(1) 1900191.

33. Fabrication and Characterization of Recombinant Silk-Elastin-Like-Protein (SELP) Fiber; E. Roberts, N. Rim, W. Huang, A. Tarakanova, J. Yeo, M. J. Buehler, D. L. Kaplan, J. Wong*; Macromolecular Bioscience, 2018/11/12, 18(12) 1800265.

32. Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk; C.H. Bowen, B. Dai, C.J. Sargent, W. Bai, P. Ladiwala, H. Feng, W. Huang, D. L. Kaplan, J. M. Galazka, F. Zhang*; Biomacromolecules, 2018/08/06, 19(9) 3853-3860.

31. Silkworm Silk-based Materials and Devices generated using Bio-nanotechnology; W. Huang, S. Ling, C. Li, F. G. Omenetto*, D. L. Kaplan*; Chemical Society Reviews, 2018/06/25, 47(17) 6486-6504.

30. Unraveling the Molecular Mechanisms of Thermo-responsive Properties of Silk-Elastin-Like Proteins by Integrating Multiscale Modeling and Experiment; J. Yeo, W. Huang, A. Tarakanova, Y. W. Zhang, D. L. Kaplan, M. J. Buehler*; Journal of Materials Chemistry B, 2018/05/03, 6(22) 3727-3734.

29. 3D freeform printing of silk fibroin; M. Rodriguez, T. Dixon, E. Cohen, W. Huang, F. Omenetto, D.L. Kaplan*; Acta biomaterialia, 2018/03/15, 71 379-387.

28. Polymorphic regenerated silk fibers assembled through bioinspired spinning; S. Ling, Z. Qin, C. Li, W. Huang, D. L. Kaplan*, M. J. Buehler*; Nature Communications, 2017/11/09, 8 1387.

27. Multiscale design and synthesis of biomimetic gradient protein/biosilica composites for interfacial tissue engineering; J. Guo, C. Li, S. Ling, W. Huang, Y. Chen, D. L. Kaplan*; Biomaterials, 2017/08/15, 145 44-55.

26. Effect of terminal modification on the molecular assembly and mechanical properties of protein-based block copolymers; M. Jacobsen, O. Tokareva, D. Ebrahimi, W. Huang, S.Ling, N. Dinjaski, D. Li, M. Simon, C. Staii, M. J. Buehler, D. L. Kaplan*, J. Wong*; Macromolecular Bioscience, 2017/06/30, 17(9) 1700095.

25. Tunable Crystallization, Degradation and Self-assembly of Recombinant Protein Block Copolymer; W. Huang, S. Krishnaji, O. Tokareva, D. L. Kaplan, P. Cebe*; Polymer, 2017/04/10, 117 107-116.

24. Design and function of biomimetic multilayer water purification membranes; S. Ling#, Z. Qin#, W. Huang, S. Chao, D. L. Kaplan*, M. J. Buehler*; Science Advances, 2017/04/05, 3(4) e1601939.

23. Modeling and Experiment Reveal Mutable Inverse Temperature Transition in Silk-Elastin-like Protein Polymers; A. Tarakanova, W. Huang, D. L. Kaplan, M. J. Buehler*; ACS Biomaterials Science & Engineering, 2017/03/16, 3 (11) 2889-2899.

22. Synergistic Integration of Experimental and Simulation Approaches for the de novo Design of Silk-based Materials; W. Huang, D. Ebrahimi, N. Dinjaski, M. J. Buehler, D. L. Kaplan*; Accounts of Chemical Research, 2017/02/13, 50 (4) 866-876.

21. Computational Smart Polymer Design based on Elastin Protein Mutability; A. Tarakanova, W. Huang, D. L. Kaplan, M. J. Buehler*; Biomaterials, 2017/01/31, 127 49-60.

20. Chemically Functionalized Silk for Human Bone Marrow-derived Mesenchymal Stem Cells Proliferation and Differentiation; K. Zheng, Y. Chen, W. Huang, Y. Lin, D. L. Kaplan*, Y. Fan*; ACS Applied Materials & Interfaces, 2016/05/13, 8 (23) 14406-14413.

19. Silk-ionomer and silk-tropoelastin hydrogels as charged three-dimensional culture platforms for the regulation of hMSCs response; R. Calabrese, N. Raia, W. Huang, C. Ghezzi, M. Simon, C. Staii, A. Weiss, D. L. Kaplan*; Journal of Tissue Engineering and Regenerative Medicine, 2016/04/06, 11 (9) 2549-2564.

18. Design of Multi-Stimuli Responsive Hydrogels using Integrated Modeling and Genetically Engineered Silk-Elastin-Like-Proteins; W. Huang, A. Tarakanova, N. Dinjaski, Q. Wang, X. Xia, Y. Chen, J. Wong, M. J. Buehler, D. L. Kaplan*; Advanced Functional Materials, 2016/04/05, 26 (23) 4113-4123.

17. Aqueous-Based Coaxial Electrospinning of Genetically Engineered Silk Elastin Core-Shell Nanofibers; J. Zhu, W. Huang, Q. Zhang, S. Ling, Y. Chen, D. L. Kaplan*; Materials, 2016/03/23, 9 (4), 221.

16. Predictive Modelling-based Design and Experiments for Synthesis and Spinning of Bioinspired Silk Fibres; S. Lin, S. Ryu, O. Tokareva, G. Gronau, M. Jacobsen, W. Huang, D. Rizzo, D. Li, C. Staii, N. Pugno, J. Wong, D. L. Kaplan, M. J. Buehler*; Nature Communications, 2015/05/28, 6 6892.

15. Lyophilized silk sponges: a versatile biomaterial platform for soft tissue engineering; J. Rnjak-Kovacina*, L. Wray, K. Burke, T. Torregrosa, J. Golinski, W. Huang, D. L. Kaplan; ACS Biomaterials Science & Engineering, 2015/02/25, 1 (4) 260-270.

14. Physical and Biological Regulation of Neuron Regenerative Growth and Network Formation on Recombinant Dragline Silks Biomaterials; B. An, M. Tang-Schomer, W. Huang, J. He, J. Jones, R. Lewis, D. L. Kaplan*; Biomaterials, 2015/02/11, 48 137-146.

13. Silk-elastin-like Protein Biomaterials for the Controlled Delivery of Therapeutics; W. Huang, A. Rollett, D. L. Kaplan*; Expert Opinion on Drug Delivery, 2014/12/5, 12 (5) 779-791.

12. Influence of Water on Protein Transitions: Morphology and Secondary Structure; W. Huang, S. Krishnaji, O. Tokareva, D. L. Kaplan, P. Cebe*; Macromolecules, 2014/11/07, 47 (22) 8107-8114.

11. Influence of Water on Protein Transitions: Thermal Analysis; W. Huang, S. Krishnaji, O. Tokareva, D. L. Kaplan, P. Cebe*; Macromolecules, 2014/11/07, 47 (22) 8098-8106.

10. Control of Silicification by Genetically Engineered Fusion Proteins: Silk-Silica Binding Peptides (SiBPs); S. Zhou, W. Huang, D. Belton, L. Simmons, C. Perry, X. Wang, D. L. Kaplan*; Acta Biomaterialia, 2014/11/04, 15 173-180.

9. Influence of Solution Parameters on Phase Diagram of Recombinant Spider Silk-Like Block Copolymers; S. Krishnaji, W. Huang, D. L. Kaplan, P. Cebe*; Macromolecular Chemistry and Physics, 2014/05/19, 215 (12) 1230-1238.

8. High throughput screening of dynamic silk-elastin-like protein biomaterials; W. Qin, X. Xia, W. Huang, Y. Lin, Q. Xu, D. L. Kaplan*; Advanced Functional Materials, 2014/04/07, 24 (27) 4303-4310.

7. Effect of sequence features on assembly of spider silk block copolymers; O. Tokareva, S. Lin, M. Jacobsen, W. Huang, D. Rizzo, D. Li, M. Simon, C. Staii, P. Cebe, J. Wong, M.J. Buehler, D. L. Kaplan*; Journal of Structural Biology, 2014/03/12, 186 (3) 412-419.

6. Tuning Chemical and Physical Crosslinks in Silk Hydrogels for Morphological Analysis and Mechanical Reinforcement; Y. Lin, X. Xia, K. Shang, R. Elia, W. Huang, P. Cebe, D. L. Kaplan*; Biomacromolecules, 2013/06/20, 14 (8) 2629-2635.

5. Charge-Tunable Autoclaved Silk-Tropoelastin Protein Alloys that Control Neuron Cell Responses; X. Hu, M. Tang-Schomer, W. Huang, X. Xia, A. Weiss, D. L. Kaplan*; Advanced Functional Materials, 2013/03/18, 23 (31) 3875-3884.

4. Thermal Analysis of Spider Silk Inspired Di-block Copolymers in the Glass Transition Region by TMDSC; W. Huang, S. Krishnaji, D. L. Kaplan, P. Cebe*; Journal of Thermal Analysis and Calorimetry, 2012/02/28, 109 (3) 1193-1201.

3. Heat Capacity of Spider Silk-Like Block Copolymers; W. Huang, S. Krishnaji, X. Hu, D. L. Kaplan, P. Cebe*; Macromolecules, 2011/06/16, 44 (13) 5299-5309.

2. Thin Film Assembly of Spider Silk-like Block Copolymers; S. Krishnaji, W. Huang, O. Tokareva, E. Kharlampieva, I. Choi, V. Tsukruk, R. Naik, P. Cebe, D. L. Kaplan*; Langmuir, 2011/01/05, 27 (3) 1000-1008.

1. Nanocomposites of Poly(vinylidene fluoride) with Multiwalled Carbon Nanotubes; W. Huang, K. Edenzon, L. Fernandez, S. Razmpour, J. Woodburn, P. Cebe*; Journal of Applied Polymer Science, 2009/11/03, 115 (6) 3238– 3248.

Book Chapter

–  Recursive Directional Ligation Approach for Recombinant Spider Silk Cloning; N. Dinjaski, W. Huang, and D. L. Kaplan, Methods in Molecular Biology on self-assembling peptides, Humana Press, New York, NY, 2018, 181-192.